Journal: Journal of Inflammation Research
Article Title: miR-6089 Alleviates Inflammation and Cell Apoptosis Through Modulating the TLR4 Pathway in Mite-Sensitized Allergic Rhinitis
doi: 10.2147/JIR.S497005
Figure Lengend Snippet: miR-6089 inhibited AR inflammation by targeting the TLR4 signal pathway. ( A ) The binding site of miR-6089 with TLR4 in human predicted by TargetScan. ( B ) Luciferase assay of HNEpC co-transfected with firefly luciferase constructs containing TLR4 wild-type (Wt) or mutated (Mut) 3’UTRs, miR-6089 mimics, and mimics NC. qRT-PCR to detect the expression of miR-6089 ( C ) and mRNA expression of TLR4 ( D ). ELISA to detect the protein expression of IL-6 ( E ), IL-8 ( F ), and TSLP ( G ). n = 3 each group. **p < 0.01, ***p < 0.001.
Article Snippet: ELISA kits for the detection of IL-6, IL-8, and TSLP protein levels were obtained from Elabscience Biotechnology Co., Ltd (Wuhan, China).
Techniques: Binding Assay, Luciferase, Transfection, Construct, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay